General Product Information: Scripps Laboratories supplies one antibody clone directed against the α-subunit and 2 antibody clones directed against the β-subunit of hCG. These antibodies may be paired to obtain 100% cross-reactivity against intact hCG with less than 1% cross-reactivity against FSH, LH, and TSH. The two β-specific antibodies can also be paired to give 100% cross-reactivity against the β-subunit. Clone BC001 has been paired with Scripps polyclonal antibody (GC099) in a diagnostic test, with esentially no cross-reactivity with LH.
Catalog No. MC085 (Part No. 90175) - clone BC005
Mouse IgG1 isotype. Specific for intact hCG and free α-hCG. Ka =4 x 1010. Sample Certificate, PDF
Catalog No. MC095 (Part No. 90176) - clone AC001
Mouse IgG1 isotype. Specific for intact hCG and free β-hCG. Ka =1 x 1010. Sample Certificate, PDF
Catalog No. MC097 (Part No. 90000) - clone BC001
Mouse IgG1 isotype. Specific for intact hCG and free β-hCG. Ka =2 x 1010. Sample Certificate, PDF
Immunoassays for hCG serum levels are useful in the detection and/or verification of normal pregnancy, as elevated levels of hCG are reportedly detectable as early as seven days after conception. In addition,
low levels of serum hCG may help diagnose ectopic pregnancy, while elevated levels of serum hCG have been reported in patients with trophoblastic disease, choriocarcinoma, and various other types of cancer.
hCG can be cleaved to yield two dissimilar subunits, α-hCG and β-hCG. The α-subunit (approximate molecular weight of 14,500 Daltons) is virtually identical to the α-subunits
of the related pituitary hormones, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), and Thyroid Stimulating Hormone (TSH). The biological activity of hCG is dependent on the distinct make-up
of the β-subunit (approximate molecular weight of 22,200 Daltons), which differs in amino acid sequence from the β-subunits of FSH, LH, and TSH.