General Product Information: TSH is isolated from human pituitary glands and provided as a powder, lyophilized from ammonium bicarbonate. Protein content is determined using the Lowry Protein Assay (BSA standard). The β- subunit is prepared from highly purified intact TSH and provided as a powder. The subunits are bulk processed and vialed by dry weight.
Catalog No. T0113 (Part No. 90243) - whole molecule
Purity: ≥ 20% by SDS-PAGE.
Activity: ≥ 1,500 mIU/mg vial by immunoassay. (1.5 IU)
Sample Certificate, MS Word
Catalog No. T0114 (Part No. 90244) - whole molecule
Purity: ≥ 90% by SDS-PAGE.
Activity: ≥ 5,000 mIU/mg vial by immunoassay. (5 IU)
Contaminants: ≤ 2% FSH, LH, and GH; ≤ 1% Prolactin
Sample Certificate, MS Word
Catalog No. T0115 (Part No. 90246) - whole molecule
Purity: ≥ 95% by SDS-PAGE.
Activity: ≥ 6,200 mIU/mg vial by immunoassay. (6.2 IU)
Contaminants: ≤ 0.1% FSH, LH, GH, and Prolactin
Sample Certificate, MS Word
Catalog No. T0214 (Part No. 90381) - α-subunit
Purity: ≥ 90% by SDS-PAGE
Sample Certificate, MS Word
Catalog No. T0314 (Part No. 90247) - β-subunit
Purity: ≥ 90% by SDS-PAGE
Sample Certificate, MS Word
Human Thyroid Stimulating Hormone (TSH) secreted by the anterior pituitary gland is a major regulating factor of thyroid hormone synthesis and secretion. It stimulates the thyroid to synthesize and release the thyroid hormones triiodothyronine (T3) and thyroxine (T4), and induces thyroglobulin production.
Serum levels of hTSH, T3, and T4 are routinely used as indicators of thyroid function. In hyperthyroid syndromes such as thyroid adenoma, nodular goiter, and the autoimmune disorder Graves' disease, hTSH serum levels are frequently well below normal, while T3 and T4 are elevated. Most cases of hypothyroidism are due to another autoimmune disease, Hashimoto's thyroiditis, in which hTSH synthesis is increased and serum levels are high, while T3 and T4 serum levels are low.
TSH can be cleaved to yield two dissimilar subunits, α-TSH and β-TSH. The α-subunit (approximate molecular weight of 13,500 Daltons) is virtually identical to the α-subunits of the related pituitary hormones, LH and FSH, and the the placental hormone hCG. The biological activity of TSH is dependent on the distinct make-up of the β-subunit (approximate molecular weight of 15,000 Daltons), which differs in amino acid sequence from the β-subunits of LH, FSH, and hCG.
Note: Monoclonal and Polyclonal Antibodies are available for β-TSH.
For antibodies to α-TSH,
please refer to the antibody section for α-hCG antibodies.