General Product Information: LH is isolated from human pituitary glands and provided as a powder, lyophilized from ammonium bicarbonate. Protein content is determined using the Lowry Protein Assay (BSA standard). The α- and β- subunits are prepared from highly purified intact LH and provided as a powder. The subunits are bulk processed and vialed by dry weight.
Catalog No. L0813 (Part No. 90152) - whole molecule
Purity: ≥ 50% by SDS-PAGE.
Activity: ≥ 5,000 IU/mg vial by immunoassay.
Sample Certificate, MS Word
Catalog No. L0814 (Part No. 90153) - whole molecule
Purity: ≥ 95% by SDS-PAGE.
Activity: ≥ 9,000 IU/mg vial by immunoassay.
Contaminants: ≤ 1% FSH, TSH; ≤ 0.5% GH, Prolactin
Sample Certificate, MS Word
Catalog No. L0815 (Part No. 90154) - whole molecule
Purity: ≥ 98% by SDS-PAGE.
Activity: ≥ 12,000 IU/mg vial by immunoassay.
Contaminants: ≤ 0.1% TSH, FSH, GH, and Prolactin
Sample Certificate, MS Word
Catalog No. L0914 (Part No. 90156) - α-subunit
Purity: ≥ 98% by SDS-PAGE
Sample Certificate, MS Word
Catalog No. L1014 (Part No. 90157) - β-subunit
Purity: ≥ 98% by SDS-PAGE
Sample Certificate, MS Word
Luteinizing Hormone (LH) belongs to a subset of glycoprotein hormones, called gonadotropins, that regulate gonadal function. Secreted by the anterior pituitary gland, LH stimulates testosterone secretion from Leydig cells in men, and stimulates the ovarian theca to produce several androgen precursors of estradiol, then promotes the formation of the corpus luteum and the subsequent production of progesterone in women.
Immunoassays for LH serum levels, along with those for follicle stimulating hormone (FSH), are useful in the evaluation of disorders of reproduction and puberty, such as hypogonadism, ovulation timing, and infertility. In addition, LH and FSH serum levels are monitored in ovulation induction and in the clinical administration of gonadotropins.
LH can be cleaved to yield two dissimilar subunits, α-LH and β-LH. Taken separately, these hormonal subunits exhibit very little of the biological activity associated with whole molecule LH. Each α-subunit (approximate molecular weight of 13,500 Daltons) is virtually identical to the α-subunits of the related pituitary hormones, FSH and TSH, and the the placental hormone hCG. The biological activity of LH is dependent on the distinct make-up of the β-subunit (approximate molecular weight of 14,500 Daltons), which differs in amino acid sequence from the β-subunits of FSH, TSH, and hCG.
Note: Monoclonal and Polyclonal Antibodies are available for β-LH.
For antibodies to α-LH,
please refer to the antibody section for α-hCG antibodies.