General Product Information: Whole molecule hCG is isolated from human pregnancy urine and provided as a powder, lyophilized from ammonium bicarbonate. Protein content is determined using the Lowry Protein Assay (BSA standard). The α- and β- subunits are prepared from highly purified intact hCG and are also provided as a powder lyophilized from ammonium bicarbonate. Protein content is determined by measuring the absorbance at 280nm (Emg/ml = 0.43 for the α-subunit and 0.21 for the β-subunit).
Catalog No. C0711 (Part No. 90052) - whole molecule
Purity: ≥ 15% by SDS-PAGE.
Activity: ≥ 2,000 IU/mg total protein by immunoassay.
Sample Certificate, MS Word
Catalog No. C0713 (Part No. 90028) - whole molecule
Purity: ≥ 80% by SDS-PAGE.
Activity: ≥ 9,000 IU/mg vial by immunoassay.
Sample Certificate, MS Word
Catalog No. C0714 (Part No. 90055) - whole molecule
Purity: ≥ 99% by SDS-PAGE.
Activity: ≥ 11,000 IU/mg vial by immunoassay.
Contaminants: ≤ 0.2% FSH, LH, TSH
Sample Certificate, MS Word
Catalog No. C0814 (Part No. 90100) - α-subunit
Purity: ≤ 2% β-hCG.
Sample Certificate, MS Word
Catalog No. C0914 (Part No. 90037) - β-subunit
Purity: ≤ 2% α-hCG.
Sample Certificate, MS Word
Human Chorionic Gonadotropin (hCG) is a sialoglycoprotein hormone secreted by the trophoblastic cells of the placenta during pregnancy. hCG production increases shortly after implantation of the fertilized ovum in the uterine wall. Although its role in the female reproductive cycle is not clear, hCG is instrumental in the maintenance of the corpus luteum at the beginning of the gestation period.
Immunoassays for hCG serum levels are useful in the detection and/or verification of normal pregnancy, as elevated levels of hCG are reportedly detectable as early as seven days after conception. In addition, low levels of serum hCG may help diagnose ectopic pregnancy, while elevated levels of serum hCG have been reported in patients with trophoblastic disease, choriocarcinoma, and various other types of cancer.
hCG can be cleaved to yield two dissimilar subunits, α-hCG and β-hCG. The α-subunit (approximate molecular weight of 14,500 Daltons) is virtually identical to the α-subunits of the related pituitary hormones, FSH, LH, and TSH. The biological activity of hCG is dependent on the distinct make-up of the β-subunit (approximate molecular weight of 22,200 Daltons), which differs in amino acid sequence from the β-subunits of FSH, LH, and TSH.
Assay Development Technical Information
See Also: Monoclonal Antibodies and Polyclonal Antibodies for hCG.