General Product Information: Native AFP is isolated from human cord serum, while the cell culture form is isolated from a human hepatoma cell line. The native form is supplied as a liquid in 10mM Sodium Phosphate, 150mM Sodium Chloride, ph 7.2 ± 0.1, containing 0.05% Sodium Azide. The cell culture form is supplied as a powder, lyophilized from 10mM Sodium Phosphate, 150mM Sodium Chloride, pH 7.4 ± 0.1. Purity is determined as indicated below. Protein concentration is determined by immunoassay.
Catalog No. A0723 (Part No. 90080) - native
Purity: ≥ 50% by Immunoassay/total protein ratio
Concentration: ≥ 1.0 mg/ml determined by immunoassay
Total protein determined by Lowry Protein Assay
Sample Certificate, MS Word
Catalog No. A0724 (Part No. 90054) - native
Purity: ≥ 95% by SDS-PAGE.
Concentration: ≥ 1.0 mg/ml determined by immunoassay
Sample Certificate, MS Word
Catalog No. A2414 (Part No. 90085) - cell culture
Purity: ≥ 95% by SDS-PAGE.
Concentration: Determined by immunoassay prior to lyophilization
Sample Certificate, MS Word
α-Fetoprotein (AFP) belongs to the α1-globulin family of human plasma proteins and contains approximately 4% carbohydrate. AFP is produced primarily in the fetal liver and can be found in fetal and maternal blood and in amniotic fluid. As part of a triple marker screening protocol with human chorionic gonadotropin and unconjugated estriol, immunoassays for maternal serum levels of AFP are useful in detecting neural tube defects and Down's Syndrome. Elevated AFP serum levels are also found in pregnant women with diabetes and Rh immunization. Furthermore, AFP serum levels may be indicative of viral hepatitis, chronic active hepatitis, ulcerative cholitis, Crohn's disease, alcoholic cirrhosis, and adenocarcinoma of the liver, lung, pancreas, stomach, and gall bladder.
Note: Monoclonal Antibodies are available for AFP.