Molecular Weight, pI & Absorbance Values of Various Proteins


Protein Approx.Mol.Wt. # of Subunits pI A280 (mg/ml)
α-Acid Glycoprotein 44,100 1 2.7 0.89
Albumin Human Serum 66,300 1 4.7 0.58
Alkaline Phosphatase 140,000 2 4.4 1.0
α-1-Antichymotrypsin 68,000 1 3.75-4.0 0.60
α-1-Antitrypsin 54,000 1 4.8 0.44
Apolipoprotein A-I 28,016 1 6.0 1.13
Apolipoprotein A-II 17,400 1 5.0 0.69
Apolipoprotein B-100 549,000 2 4.7 0.77
Apolipoprotein C-I 6,605 1 6.5  
Apolipoprotein C-II 8,824 1 5.0  
Apolipoprotein C-III 8,750 1 4.8 - 5.1  
Apolipoprotein E 34,200 1 5.7 - 6.2  
Apolipoprotein H 54,000 1 5.6 - 6.4  
Avidin 68,300 4 10 1.54
Bilirubin Conjugate 844.7 1    
C-Peptide 3,019 1    
C-Peptide, tyrosylated 3,184 1    
C-Reactive Protein 118,000 5 7.4 1.7
Carcinoembryonic Antigen 180,000 1 3.7  
hCG 36,700 2 4.9 0.36
CKBB 82,000 2 5.0 - 7.5 0.88
CKMB 82,000 2 5.0 - 7.5 0.88
CKMM 82,000 2 5.0 - 7.5 0.88
Elastase 29,500 1 10.0 0.985
Ferritin 450,000 24 5.45  
α-Fetoprotein 70,000 1 4.75 0.53
Folate Binding Protein 40,000 1 7.2 0.40
FSH, human 35,500 2 5.5 0.71
GH, human 21,700 1 4.9 0.93
HBsAg, ad Subtype       3.73
HBsAg, ay Subtype       3.73
IgA, human 162,000 4   1.34
IgE, human 190,000 4   1.53
IgG, goat 144,000 4   1.30
IgG, human 153,000 4 6.6 - 7.2 1.38
IgG, mouse 155,000 4 ca. 7.0 1.4
IgM, human 950,000 20 5.5 - 7.4 1.33
Intrinsic Factor, porcine 75,000     0.14
Keratin, epidermal 56,000 - 67,000 1 5.3 - 8.0  
Lactogen, placental 21,000 1   0.91
Lipoprotein (a) 1,000 - 1,500K 1    
LH, human 28,500 2 7.4  
α-2-Macroglobulin 726,000 4    
α-1-Microglobulin 27,150 1   1.74
β-2-Microglobulin 11,800 1 5.6 1.68
Myoglobin 17,510 1   1074
Myosin Light Chain 1 21,600 1 5.05  
Neuron Specific Enolase 78,000 2 4.7 0.65
Peroxidase 45,900 1 4.0 - 11.0 0.70
Ovalbumin 45,000 1 4.5 7.5
Prolactin, human 22,800 1 5.7 0.9
Prostate Specific Antigen 33,500 1 6.0 1.86
Prostatic Acid Phosphatase 102,000 2 4.9 1.44
Protein A 32,000 1   0.2
Retinol Binding Protein 21,400 1 4.7 1.87
SHBG 90,000 2    
Streptavidin 60,000 4 6.0 3.4
Thyroglobulin 665,500 2 4.49 1.05
TSH, human 25,000 2   0.99
Thyroxine Binding Globulin 58,000 1 4.0 0.89
Troponin I 22,500 1    
Troponin T 37,000 - 39,000 1    
Trypsin 22,000 1 10.1 1.45

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