NSE - Neuron Specific Enolase

General Product Information

NSE is isolated from human brain and supplied as a liquid in 100mM Tris-HCl, 5mM Magnesium Sulfate, 250mM Potassium Chloride, adjusted to pH 5.1 ± 0.1 using Sodium Phosphate. Protein concentration is determined by absorbance at A280 using an extinction coefficient of 0.895 (Emg/ml = 0.895). Activity is reported by an in-house assay, where one unit causes the formation of 1µmole of phosphoenolpyruvate per minute at 25°C, pH 6.8.

Neuron Specific Enolase (NSE) is the most abundant form of the glycolytic enolase found in adult neurons and is thought to serve as a growth factor in neurons. Of the three enolase subunits (α, β, and γ), NSE is a dimer composed of two γ subunits.

NSE is useful in studying neuronal differentiation and is, therefore, a valuable tool for visualizing the entire neuron and endocrine systems. Serum levels of NSE have been associated with such disease states as Alzheimer's, Huntington's Chorea, neuroblastoma, head trauma, neuroendocrine malignancies, and small cell carcinomas of the lung.

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